@article{oai:tsc.repo.nii.ac.jp:00002072,
 author = {渡邉, 悟 and 牛澤, 良美 and 安井, 恒男 and ワタナベ, サトル and ウシザワ, ヨシミ and ヤスイ, ツネオ and WATANABE, SATORU and USHIZAWA, YOSHIMI and YASUI, TSUNEO},
 journal = {聖徳栄養短期大学紀要},
 month = {Dec},
 note = {P(論文), "Glucose C II - Test Wako" (Glucose Oxidase (GOD) Reagent) reacts glucose well, and is sensitive against xylose and mannose (M_1). Using this reagent and β-1, 4-mannobiose (M_2), Aspergillus niger β-mannosidase activily could be measured. The enzyme hydrolyzed M_2 to produce M_1, and no transfer product was detected in the thin-layer chromatography. Optimal pH of the enzyme using M_2 was measured to be the same around pH 3-4 as using p-nitrophenyl β-D-mannopyranoside (PNPM). The enzyme activity measured by the developed method seemed to be identical with the activity using PNPM as substrate. This result indicate that β-mannosidase activity can be measured by the developed method using M_2 instead of PNPM.},
 pages = {8--12},
 title = {β-1.4-マンノビオースを用いたβ-マンノシダーゼ活性測定法},
 volume = {23},
 year = {1992}
}